DNAJB1 Protein (GST tag)
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- Target See all DNAJB1 Proteins
- DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This DNAJB1 protein is labelled with GST tag.
- Application
- Western Blotting (WB)
- Supplier Product No.
- 009-001-e57s
- Supplier
- Rockland
- Purpose
- HSP40 recombinant protein-GST fusion protein
- Purification
- Recombinant full-length human HSP40 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.
- Purity
- >90%
- Top Product
- Discover our top product DNAJB1 Protein
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- Application Notes
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Western_Blot_Dilution: User Optimized
Application_Note: HSP40 Protein is suitable for use in Western Blot. Expect a band approximately ~65 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 μg/μL
- Buffer
- HSP40 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))
- Alternative Name
- DNAJB1 (DNAJB1 Products)
- Background
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Synonyms: DNAJB1, HSPF1, DnaJ homolog subfamily B member 1, DnaJ protein homolog 1, Heat shock 40 kDa protein 1, HSP40, Heat shock protein 40, Human DnaJ protein 1, hDj-1
Background: HSP40 is a member of heat shock proteins (HSPs) which are synthesized in cells in response to heat shock and other metabolic stresses and provide a transient state of thermotolerance. HSP40 is localized faintly throughout the cell in non-heat-shocked cells and accumulates in the nuclei and nucleoli in heat-shocked cells (1). The intracellular localization of HSP40 is very similar to that of HSP70. Overexpression of HSP40 reduces aggregate formation and suppresses apoptosis in a neuronal cell model of spinal and bulbar muscular atrophy (inherited neurodegenerative diseases caused by polyglutamine expansion) (2). HSP40 is ideal for investigators involved in Signaling Proteins, Cell Stress & Chaperone Proteins, Cancer, Cellular Stress, and Inflammation research.
- NCBI Accession
- NM_006145
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