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DNAJB1 Protein (GST tag)

DNAJB1 Origin: Human Host: Escherichia coli (E. coli) Recombinant >90% WB
Rockland
Catalog No. ABIN6699783
Supplier Product No.: 009-001-e57s
  • Target See all DNAJB1 Proteins
    DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))
    Protein Type
    Recombinant
    Origin
    • 8
    • 1
    • 1
    Human
    Source
    • 7
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This DNAJB1 protein is labelled with GST tag.
    Application
    Western Blotting (WB)
    Supplier Product No.
    009-001-e57s
    Supplier
    Rockland
    Purpose
    HSP40 recombinant protein-GST fusion protein
    Purification
    Recombinant full-length human HSP40 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.
    Purity
    >90%
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  • Application Notes

    Western_Blot_Dilution: User Optimized

    Application_Note: HSP40 Protein is suitable for use in Western Blot. Expect a band approximately ~65 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.2 μg/μL
    Buffer
    HSP40 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
    Storage
    -80 °C
    Storage Comment
    Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
    Expiry Date
    12 months
  • Target
    DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))
    Alternative Name
    DNAJB1 (DNAJB1 Products)
    Background

    Synonyms: DNAJB1, HSPF1, DnaJ homolog subfamily B member 1, DnaJ protein homolog 1, Heat shock 40 kDa protein 1, HSP40, Heat shock protein 40, Human DnaJ protein 1, hDj-1

    Background: HSP40 is a member of heat shock proteins (HSPs) which are synthesized in cells in response to heat shock and other metabolic stresses and provide a transient state of thermotolerance. HSP40 is localized faintly throughout the cell in non-heat-shocked cells and accumulates in the nuclei and nucleoli in heat-shocked cells (1). The intracellular localization of HSP40 is very similar to that of HSP70. Overexpression of HSP40 reduces aggregate formation and suppresses apoptosis in a neuronal cell model of spinal and bulbar muscular atrophy (inherited neurodegenerative diseases caused by polyglutamine expansion) (2). HSP40 is ideal for investigators involved in Signaling Proteins, Cell Stress & Chaperone Proteins, Cancer, Cellular Stress, and Inflammation research.

    NCBI Accession
    NM_006145
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