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EIF4E Protein (GST tag)

EIF4E Origin: Human Host: Escherichia coli (E. coli) Recombinant >85% WB
Rockland
Catalog No. ABIN6699816
Supplier Product No.: 009-001-r87s
  • Target See all EIF4E Proteins
    EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))
    Protein Type
    Recombinant
    Origin
    • 7
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 6
    • 5
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This EIF4E protein is labelled with GST tag.
    Application
    Western Blotting (WB)
    Supplier Product No.
    009-001-r87s
    Supplier
    Rockland
    Purpose
    EIF4E recombinant protein GST fusion protein
    Purification
    Recombinant full-length human EIF4E was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.
    Purity
    >85%
    Top Product
    Discover our top product EIF4E Protein
  • Application Notes

    Western_Blot_Dilution: User Optimized

    Other: Kinase Assay-User Optimized

    Application_Note: EIF4E Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~ 51 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.2 μg/μL
    Buffer
    EIF4E Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
    Storage
    -80 °C
    Storage Comment
    Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
    Expiry Date
    12 months
  • Target
    EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))
    Alternative Name
    EIF4E (EIF4E Products)
    Background

    Synonyms: CBP, EIF4F, EIF4E1, EIF4EL1, MGC111573, Eukaryotic translation initiation factor 4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein

    Background: Eukaryotic Translation Initiation Factor 4E (EIF4E), plays a role in translation initiation where it binds the 5 m7G cap found on mRNAs. EIF4E governs cell cycle progression and cellular proliferation by coordinately the expression of several genes at the post-transcriptional level. EIF4E functions as a central node of an RNA regulon, which plays an essential role in normal differentiation and development (1). The significant association of EIF4E with VEGF and cyclin D1 in multiple tumors supports a role for EIF4E in translational regulation of proteins related to angiogenesis and growth. The overexpression of EIF4E is involved in the malignant progression of human breast cancer (2). EIF4E Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Angiogenesis, Cancer, Cell Cycle, Cellular Stress, ERK/MAPK Pathway, Inflammation, and Neurobiology research.

    NCBI Accession
    NM_001968
    Pathways
    BCR Signaling
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