FANCL Protein (GST tag)

Catalog No. ABIN6699824

Supplier Product No.: 009-001-r93s

Product Details

Target: FANCL (Fanconi Anemia, Complementation Group L (FANCL))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Purification tag / Conjugate: This FANCL protein is labelled with GST tag.

Application: Western Blotting (WB)

Supplier Product No.: 009-001-r93s

Supplier: Rockland

Purpose: FANCL recombinant protein-GST fusion protein

Purification: Recombinant full-length human FANCL was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >75% by densitometry.

Purity: >75%

Application Details

Application Notes:

Application Note: FANCL Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. FANCL Protein is suitable for use in Western Blot. Expect a band approximately ~ 67 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 μg/μL

Buffer: FANCL Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: FANCL (Fanconi Anemia, Complementation Group L (FANCL))

Alternative Name: FANCL (FANCL Products)

Background:

Synonyms: FAAP43, PHF9, POG, E3 ubiquitin-protein ligase FANCL, Fanconi anemia group L protein, Fanconi anemia-associated polypeptide of 43 kDa

Background: FANCL or Fanconi anemia complementation group L is a member of the Fanconi anemia complementation group. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. FANCL is crucial in the FA pathway as the catalytic subunit required for monoubiquitination of FANCD2 (1). UBE2W interacts with FANCL and regulates the monoubiquitination of Fanconi anemia protein FANCD2 (2). FANCL Protein is ideal for investigators involved in Signaling Proteins, Ubiquitin Proteins, Angiogenesis, Apoptosis/Autophagy, Cancer, Cell Cycle, Cellular Stress, Inflammation, Invasion/Metastasis, and Neurobiology research.

NCBI Accession: NM_018062

Pathways: DNA Damage Repair