GBR2 protein (GST tag)
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- Target
- GBR2
- Protein Type
- Recombinant
- Origin
- Human
- Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- GST tag
- Application
- Western Blotting (WB)
- Supplier Product No.
- 009-001-r99s
- Supplier
- Rockland
- Purpose
- GRB2 recombinant protein-GST fusion protein
- Purification
- Recombinant full-length human GRB2 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.
- Purity
- >95%
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- Application Notes
-
Western_Blot_Dilution: User Optimized
Application_Note: GRB2 Protein is suitable for use in Western Blot. Expect a band approximately ~49 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.2 μg/μL
- Buffer
- GRB2 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- GBR2
- Background
-
Synonyms: ASH, Grb3-3, MST084, MSTP084, EGFRBP-GRB2, Growth factor receptor-bound protein 2, Adapter protein GRB2, Protein Ash, SH2/SH3 adapter GRB2
Background: GRB2 is an adaptor protein that is the homolog of the Sem5 gene of C. elegans and Drk gene of Drosophila. GRB2 protein contains one SH2 domain and two SH3 domains and plays a role in growth factor-mediated signal transduction. GRB2 forms a complex with activated EGFR and the RAS-specific guanine nucleotide exchange factor SOS1 thereby mediating the growth factor-induced activation of RAS (1). GRB2 has been implicated in growth factor regulation of the cytoskeleton and DNA synthesis. DAB2 also binds to GRB2 and competes with SOS for GRB2 binding. GRB2 interacts with the hepatitis C virus nonstructural 5A (NS5A) protein and inhibits the activation of ERK1 and ERK2 by EGF (2). GRB2 Protein is ideal for investigators involved in Signaling Proteins, Adaptor Proteins, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, JAK/STAT Pathway, and Neurobiology research.
- NCBI Accession
- NM_002086
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