TNNI1 Protein (His tag)

Catalog No. ABIN6700744

Supplier Product No.: 009-001-u22s

Product Details

Target: TNNI1 (Slow Skeletal Troponin I (TNNI1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This TNNI1 protein is labelled with His tag.

Application: Western Blotting (WB)

Supplier Product No.: 009-001-u22s

Supplier: Rockland

Purpose: Troponin1 recombinant protein-HIS Epitope

Purification: Recombinant full-length human Troponin1 protein was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >75% by densitometry.

Purity: >75%

Application Details

Application Notes:

Western_Blot_Dilution: User Optimized

Application_Note: Troponin1 Protein is suitable for use in Western Blot. Expect a band approximately ~24 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: Troponin1 Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNNI1 (Slow Skeletal Troponin I (TNNI1))

Alternative Name: TNNI1 (TNNI1 Products)

Background:

Synonyms: TNNI1, DKFZp451O223, SSTNI, TNN1, Troponin I, slow skeletal muscle, Troponin I, slow-twitch isoform

Background: Troponin1 is a member of the tropomyosin family and regulates the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin1 is part of a complex with troponin T and troponin C in which Troponin1 acts as an inhibitory subunit that blocks actin-myosin interactions and thereby mediates striated muscle relaxation (1). Troponin1 is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The Troponin1 protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes (2). Troponin1 Protein is ideal for investigators involved in Signaling Proteins, Microtubule/Actin Associated Proteins, and Cardiovascular Disease research.

NCBI Accession: NM_003281