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SARS-CoV-2 NSP1 Protein (His tag)

NSP1 Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: Escherichia coli (E. coli) Recombinant >90 % as determined by SDS-PAGE. SDS
Catalog No. ABIN6952638
  • Target See all SARS-CoV-2 NSP1 (NSP1) products
    SARS-CoV-2 NSP1 (NSP1) (SARS-CoV-2 Host Translation Inhibitor Nsp1 (NSP1))
    Protein Type
    Recombinant
    Origin
    SARS Coronavirus-2 (SARS-CoV-2)
    Source
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This SARS-CoV-2 NSP1 protein is labelled with His tag.
    Application
    SDS-PAGE (SDS)
    Purpose
    SARS-CoV-2 (COVID-19) NSP1 Protein, His Tag
    Sequence
    AA 1-180
    Characteristics
    SARS-CoV-2 NSP1, His Tag is expressed from E.coli cells. It contains AA Met 1 - Gly 180 (Accession # YP_009725297.1). Predicted N-terminus: Met
    This protein carries a polyhistidine tag at the C-terminus.
    Purity
    >90 % as determined by SDS-PAGE.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1.0 EU per μg by the LAL method.
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, Arginine, pH 7.4
    Storage
    4 °C,-20 °C,-80 °C
    Storage Comment
    For long term storage, the product should be stored at lyophilized state at -20°C or lower.
    This product is stable after storage at:
    4-8°C for 12 months in lyophilized state,
    -70°C for 3 years under sterile conditions after reconstitution.
  • Target
    SARS-CoV-2 NSP1 (NSP1) (SARS-CoV-2 Host Translation Inhibitor Nsp1 (NSP1))
    Alternative Name
    SARS-CoV-2 Host Translation Inhibitor Nsp1 (NSP1 Products)
    Synonyms
    pp1ab Protein, ORF1a polyprotein;ORF1ab polyprotein Protein, ORF1ab Protein
    Target Type
    Viral Protein
    Background
    The viral nonstructural protein 1 (nsP1) is the only membrane-associated protein that anchors the replication complex to the cellular membranes. NSP1 inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.
    Molecular Weight
    21.7 kDa
    NCBI Accession
    YP_009725297
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