SARS-CoV-2 NSP1 Protein (His tag)
NSP1
Origin: SARS Coronavirus-2 (SARS-CoV-2)
Host: Escherichia coli (E. coli)
Recombinant
>90 % as determined by SDS-PAGE.
SDS
1 image
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- Target See all SARS-CoV-2 NSP1 (NSP1) products
- SARS-CoV-2 NSP1 (NSP1) (SARS-CoV-2 Host Translation Inhibitor Nsp1 (NSP1))
- Protein Type
- Recombinant
- Origin
- SARS Coronavirus-2 (SARS-CoV-2)
- Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This SARS-CoV-2 NSP1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purpose
- SARS-CoV-2 (COVID-19) NSP1 Protein, His Tag
- Sequence
- AA 1-180
- Characteristics
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SARS-CoV-2 NSP1, His Tag is expressed from E.coli cells. It contains AA Met 1 - Gly 180 (Accession # YP_009725297.1). Predicted N-terminus: Met
This protein carries a polyhistidine tag at the C-terminus. - Purity
- >90 % as determined by SDS-PAGE.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- PBS, Arginine, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
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For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
4-8°C for 12 months in lyophilized state,
-70°C for 3 years under sterile conditions after reconstitution.
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- Target
- SARS-CoV-2 NSP1 (NSP1) (SARS-CoV-2 Host Translation Inhibitor Nsp1 (NSP1))
- Alternative Name
- SARS-CoV-2 Host Translation Inhibitor Nsp1 (NSP1 Products)
- Synonyms
- pp1ab Protein, ORF1a polyprotein;ORF1ab polyprotein Protein, ORF1ab Protein
- Target Type
- Viral Protein
- Background
- The viral nonstructural protein 1 (nsP1) is the only membrane-associated protein that anchors the replication complex to the cellular membranes. NSP1 inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.
- Molecular Weight
- 21.7 kDa
- NCBI Accession
- YP_009725297
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