OV16 (AA 17-197) protein (AVI tag,GST tag,Biotin)
-
- Target
- OV16
- Protein Type
- Recombinant
- Protein Characteristics
- AA 17-197
- Origin
- Onchocerca volvulus
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- AVI tag,GST tag,Biotin
- Specificity
- Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- Characteristics
- Biotinylated Onchocerca volvulus OV16 protein, Avi,GST Tag
- Purity
- >95 % as determined by SDS-PAGE.
- Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
-
OV16 (AA 17-197) protein (GST tag)
Origin: Onchocerca volvulus Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE.
OV16 (AA 17-197) protein (His tag)Origin: Onchocerca volvulus Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
OV16 (AA 17-197) protein (His tag)Origin: Onchocerca volvulus Host: Yeast Recombinant > 90 % SDS
-
- Comment
-
Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Buffer
- PBS, pH 7.4
- Storage
- -20 °C
-
- Target
- OV16
- Background
- The OV16 antigen, localizes to the hypodermis, cuticle, and uterus of female O. volvulus is regarded as a highly sensitive and specific antigen for detection of IgG4 in people with prior exposure to onchocerciasis. It has an open reading frame that encodes a 152- amino acid polypeptide containing a 16-amino acid putative signal peptide. Enzyme-linked Immunosorbent Assay serological testing of OV-16 antigen is a diagnostic tool for determining effective elimination of the parasite.
- Molecular Weight
- 48.6 kDa
-
