cGMP-Dependent 3',5'-Cyclic Phosphodiesterase (PDE2A) (AA 215-900) protein (His tag)
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- Target
- cGMP-Dependent 3',5'-Cyclic Phosphodiesterase (PDE2A)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 215-900
- Origin
- Human
- Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- His tag
- Purpose
- Recombinant Human PDE2A/CGS-PDE Protein (aa 215-900, His Tag)
- Sequence
- Glu 215-His 900
- Characteristics
- A DNA sequence encoding the amino acids (Glu 215-His 900) of human PDE2A (O00408-1) was fused with a polyhistidine tag at the C-terminus.
- Purity
- > 90 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- cGMP-Dependent 3',5'-Cyclic Phosphodiesterase (PDE2A)
- Alternative Name
- PDE2A/CGS-PDE
- Background
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Background: cGMP-dependent 3',5'-cyclic phosphodiesterase, also known as cyclic GMP-stimulated phosphodiesterase and PDE2A, is a peripheral membrane protein which belongs to the cyclic nucleotide phosphodiesterase family and PDE2 subfamily. Phosphodiesterases (PDEs) comprise a family of enzymes that regulate the levels of cyclic nucleotides, key second messengers that mediate a diverse array of functions. Phosphodiesterases (PDEs) modulate signaling by cyclic nucleotides in diverse processes such as cardiac contractility, platelet aggregation, lipolysis, glycogenolysis, and smooth muscle contraction. PDE2A is an evolutionarily conserved cGMP-stimulated cAMP and cGMP PDE. PDE2A contains two GAF domains. PDE2A is expressed in brain and to a lesser extent in heart, placenta, lung, skeletal muscle, kidney and pancreas. PDE2A is a cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. PDE2A is involved in the regulation of blood pressure and fluid homeostasis by the atrial natriuretic peptide (ANP), making PDE2-type enzymes important targets for drug discovery.
Synonym: CGS-PDE;cGSPDE;PDE2A1;PED2A4
- Molecular Weight
- 80.0 kDa
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