HDAC8 Protein (GST tag)

Catalog No. ABIN7317739

Product Details

Target: HDAC8 (Histone Deacetylase 8 (HDAC8))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This HDAC8 protein is labelled with GST tag.

Purpose: Recombinant Human HDAC8/HDACL1 Protein (GST Tag)

Sequence: Met 1-Val 377

Characteristics: A DNA sequence encoding the full length of human HDAC8 isoform 1 (NP_060956.1) (Met 1-Val 377) was expressed, fused with the GST tag at the C-terminus.

Purity: > 88 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 10 % glycerol, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: HDAC8 (Histone Deacetylase 8 (HDAC8))

Alternative Name: HDAC8/HDACL1 (HDAC8 Products)

Background:

Background: Histone deacetylase 8, also known as HDAC8 and HDACL1, is a nucleus and cytoplasm protein which belongs to the histone deacetylase family and HD type 1 subfamily. Histone deacetylases (HDACs) are a growing family of enzymes implicated in transcriptional regulation by affecting the acetylation state of core histones in the nucleus of cells. HDAC8 / HDACL1 is weakly expressed in most tissues. It expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney. HDAC8 / HDACL1 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones ( H2A, H2B, H3 and H4 ). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC8 / HDACL1 may play a role in smooth muscle cell contractility. HDAC8 / HDACL1 may be a potential drug target for neuroblastoma differentiation therapy using selective inhibitors, avoiding unspecific side effects.

Synonym: CDA07;CDLS5;HD8;HDACL1;MRXS6;RPD3;WTS

Molecular Weight: 68 kDa

NCBI Accession: NP_060956

Pathways: Cellular Glucan Metabolic Process