PCSK1 Protein (His tag)

Catalog No. ABIN7197525

This Recombinant PCSK1 protein is produced in HEK-293 Cells.

Quick Overview for PCSK1 Protein (His tag) (ABIN7197525)

Target: PCSK1 (Proprotein Convertase Subtilisin/kexin Type 1 (PCSK1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This PCSK1 protein is labelled with His tag.

Purpose: Recombinant Human PCSK1/NEC1 Protein (His Tag)

Sequence: Met 1-Arg 617

Characteristics: A DNA sequence encoding the human PCSK1 (NP_000430.3) (Met 1-Arg 617) was fused with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 25 mM Tris, 150 mM NaCl, pH 7.5

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: PCSK1 (Proprotein Convertase Subtilisin/kexin Type 1 (PCSK1))

Alternative Name: PCSK1/NEC1

Background:

Background: Neuroendocrine convertase 1, also known as Prohormone convertase 1, Proprotein convertase 1, PCSK1 and NEC1, is an enzyme which belongs to the peptidase S8 family and Furin subfamily. PCSK1 is an enzyme that performs the proteolytic cleavage of prohormones to their intermediate (or sometimes completely cleaved) forms. It is present only in neuroendocrine cells such as brain, pituitary and adrenal, and most often cleaves after a pair of basic residues within prohormones but can occasionally cleave after a single arginine. It binds to a protein known as proSAAS, which also represents its endogenous inhibitor. PCSK1 is involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. PCSK1 substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin. Defects in PCSK1 are the cause of proprotein convertase 1 deficiency (PC1 deficiency). PC1 deficiency is characterized by obesity, hypogonadism, hypoadrenalism, reactive hypoglycemia as well as marked small-intestinal absorptive dysfunction. It is due to impaired processing of prohormones.

Synonym: BMIQ12;NEC1;PC1;PC3;SPC3

Molecular Weight: 57.4 kDa

NCBI Accession: NP_000430

Pathways: Peptide Hormone Metabolism