PPM1A Protein (His tag)
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- Target See all PPM1A Proteins
- PPM1A (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A (PPM1A))
- Protein Type
- Recombinant
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PPM1A protein is labelled with His tag.
- Purpose
- Recombinant Mouse PPM1A Protein (His Tag)
- Sequence
- Met 1-Trp 382
- Characteristics
- A DNA sequence encoding the mouse PPM1A (NP_032936.1) (Met 1-Trp 382) was expressed, with a polyhistide tag at the C-terminus.
- Purity
- > 98 % as determined by SDS-PAGE
- Top Product
- Discover our top product PPM1A Protein
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Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A (PPM1A) (AA 1-382) protein (GST tag)
PPM1A Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A (PPM1A) (Transcript Variant 1) protein (His tag)PPM1A Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A (PPM1A) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PPM1A Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile 10 mM HEPES, 500 NaCl, pH 7.5
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- PPM1A (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A (PPM1A))
- Alternative Name
- PPM1A (PPM1A Products)
- Background
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Background: Protein phosphatase 1A (PPM1A / PP2CA) is an enzyme belonging to the PP2C family of Ser / Thr protein phosphatases. Members of PP2C family are negative regulators of cell stress response pathways and the MAP kinases and MAP kinase kinases. It has also been demonstrated to inhibit the activation of p38 and JNK kinase cascades. PPM1A dephosphorylates and promotes nuclear export of TGFβ-activated Smad2/3. Ectopic expression of PPM1A abolishes TGFβ-induced antiproliferative and transcriptional responses, whereas depletion of PPM1A enhances TGFβ signaling in mammalian cells. It has been demonstrated that PPM1A / PP2CA, through dephosphorylation of Smad2/3, plays a critical role in terminating TGFβ signaling. Overexpression of PPM1A is reported to activate the expression of the tumor suppressor gene TP53 / p53, which leads to cell apoptosis.
Synonym: 2310003C21Rik;2900017D14Rik;AI427932;AU017636;MMPa-2;MPPa-1
- Molecular Weight
- 43.3 kDa
- NCBI Accession
- NP_032936
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