SERPINB9 Protein (His tag)

Catalog No. ABIN7317334

Product Details

Target: SERPINB9 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 9 (SERPINB9))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This SERPINB9 protein is labelled with His tag.

Purpose: Recombinant Human SERPINB9/PI-9 Protein (Baculovirus, His Tag)

Sequence: Glu 2-Pro 376

Characteristics: A DNA sequence encoding the human SERPINB9 (P50453) (Glu 2-Pro 376) was expressed, with a polyhistidine tag at the N-terminus.

Purity: > 97 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: SERPINB9 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 9 (SERPINB9))

Alternative Name: SERPINB9/PI-9 (SERPINB9 Products)

Background:

Background: SerpinB9; also known as Cytoplasmic antiproteinase 3; CAP-3; Peptidase inhibitor 9; SERPINB9 and PI-9; is a cytoplasm protein which belongs to the serpin family and Ov-serpin subfamily. Serpin-B9 ( CAP-3 / PI-9 ) is the only known human intracellular inhibitor of granzyme B (GrB); the effector molecule in immunity against cytomegalovirus (CMV) and in renal allograft rejection. Serpin-B9 and SPI-6 expression in immune-privileged cells; APCs; and CTLs protects these cells against the actions of granzyme B; and when expressed in tumor cells or virally infected hepatocytes; confers resistance to killing by CTL and NK cells. Expression of increasing levels of Serpin-B9 ( CAP-3 / PI-9 ) in target cells may progressively inhibit immune surveillance by blocking NK and CTL-induced cytotoxicity through the perforin / granzyme pathway and then through the Fas / FasL pathway. Serpin-B9 ( CAP-3 / PI-9 ) is selectively up-regulated in hepatocytes in response to infiltration of the liver by NK cells that express perforin and enzymatically active granzyme B. Upregulated expression of Serpin-B9 ( CAP-3 / PI-9 ) in NSCLC cells may serve to protect them from apoptosis induced by GrB.

Synonym: Cytoplasmic antiproteinase 3;Peptidase inhibitor 9;CAP3;PI-9;Serpin B9

Molecular Weight: 44.5 kDa

UniProt: P50453