Respiratory Syncytial Virus Glycoprotein (RSV G) protein (His tag)

Catalog No. ABIN7198762

Product Details

Target: Respiratory Syncytial Virus Glycoprotein (RSV G)

Protein Type: Recombinant

Origin: Respiratory Syncytial Virus (RSV)

Source: HEK-293 Cells

Purification tag / Conjugate: His tag

Purpose: Recombinant RSV (A, rsb1734) glycoprotein G / RSV-G Protein (95% Homology) (His Tag)

Sequence: Asn 66-Arg 297

Characteristics: A DNA sequence encoding the glycoprotein G extracellular domain (Asn 66-Arg 297) of human respiratory syncytial virus A (95 % homologous with strain rsb1734) (P27022-1) was expressed, with a C-terminal polyhistidine tag.

Purity: > 90 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Application Details

Comment:

80-90 kDa

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Respiratory Syncytial Virus Glycoprotein (RSV G)

Alternative Name: RSV Glycoprotein

Background:

Background: Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. It is classified within the genus pneumovirus of the family paramyxoviridae. Like other members of the family, HRSV has two major surface glycoproteins (G and F) that play important roles in the initial stages of the infectious cycle. HRSV G protein is a type II glycoprotein of 289-299 amino acids (depending on the virus strain) with a signal/anchor hydrophobic domain and is extensively modified by the addition of both N-and O-linked oligosaccharides to achieve the mature form of 8-9 kDa. The C-terminal ectodomain of the G protein has a central region and four cysteines which are conserved in all HRSV isolates and have been proposed as the putative receptor binding site. The G protein mediates attachment of the virus to the host cell membrane by interacting with heparan sulfate, initiating the infection. As similar to mucins in amino acid compositions, the RSV G protein can interact with host CX3CR1, the receptor for the CX3C chemokine fractalkine, and thus modulates the immune response and facilitate infection. Secreted glycoprotein G helps RSV escape antibody-dependent restriction of replication by acting as an antigen decoy and by modulating the activity of leukocytes bearing Fcgamma receptors. Unlike the other paramyxovirus attachment proteins, HRSV-G lacks both neuraminidase and hemagglutinating activities.

Synonym: G Protein, RSV, Human respiratory syncytial virus

Molecular Weight: 26.7kDa