beta-Glucuronidase/Uida/Beta-Gus protein (His tag)
Origin: E. coli
Host: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by reducing SDS-PAGE.
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- Target
- beta-Glucuronidase/Uida/Beta-Gus
- Protein Type
- Recombinant
- Origin
- E. coli
- Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Purpose
- Recombinant E.coli Beta-Glucuronidase/Uida/Beta-Gus Protein (His Tag)
- Sequence
- Met1-Gln603
- Characteristics
- Recombinant E.coli Beta-Glucuronidase is produced by our E.coli expression system and the target gene encoding Met1-Gln603 is expressed with a 6His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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- Comment
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72 kDa
- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- beta-Glucuronidase/Uida/Beta-Gus
- Background
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Background: Beta-D-glucuronidase from E.coli is a ?highly specific enzyme in hydrolyzing glucuronides. Beta-D-glucuronidase (GLUase) activity can be measured as the rate of production of fluorescent methylumbelliferone (MU), resulting from the hydrolysis of the substrate 4-methylumbelliferyl-β-d-glucuronide (MUGLU), which is an effective and rapid method for detection and verification of E. coli in food, water, and environmental samples. High purity recombinant beta-Glucuronidase is used in research, biochemical enzyme assays and in vitro diagnostic analysis, detecting a wide range of drugs such as opioids, benzodiazepines, steroids, cannabinoids, and others.
Synonym: Beta-glucuronidase,uidA,GUS
- Molecular Weight
- 69.9 kDa
- UniProt
- P05804
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