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CD96 Protein (CD96) (AA 22-503) (His tag)

CD96 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Catalog No. ABIN7274264
  • Target See all CD96 Proteins
    CD96
    Protein Type
    Recombinant
    Protein Characteristics
    AA 22-503
    Origin
    • 14
    • 3
    • 1
    • 1
    • 1
    Human
    Source
    • 12
    • 2
    • 2
    • 2
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This CD96 protein is labelled with His tag.
    Purpose
    Human CD96/TACTILE Protein
    Sequence
    Val22-Met503
    Characteristics
    Recombinant Human CD96/TACTILE Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val22-Met503.
    Purity
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Human CD96, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD96 Antibody, hFc Tag with the EC50 of 0.30μg/ml determined by ELISA. The affinity constant of 0.958 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    Storage
    -20 °C,-80 °C
    Storage Comment
    -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    CD96
    Alternative Name
    CD96 (CD96 Products)
    Background
    The receptors CD96 and TIGIT are expressed on the surface of T and natural killer (NK) cells, and recent studies suggest both play important inhibitory roles in immune function. CD96 has been shown to modulate immune cell activity in mice, with Cd96-/- mice displaying hypersensitive NK-cell responses to immune challenge and significant tumor resistance. The counterbalance between the putative inhibitory CD96 and TIGIT receptors and the activating receptor, CD226, offers unique strategies for immuno-oncology drug development.
    Molecular Weight
    54.7 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.
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