CD96 Protein (CD96) (AA 22-503) (His tag)
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- Target See all CD96 Proteins
- CD96
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-503
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD96 protein is labelled with His tag.
- Purpose
- Human CD96/TACTILE Protein
- Sequence
- Val22-Met503
- Characteristics
- Recombinant Human CD96/TACTILE Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val22-Met503.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD96, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD96 Antibody, hFc Tag with the EC50 of 0.30μg/ml determined by ELISA. The affinity constant of 0.958 μM as determined in SPR assay (Biacore T200). See testing image for detail.
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CD96 (CD96) (AA 22-502) protein (His tag)
CD96 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD96 (CD96) (AA 22-503) protein (mFc Tag)CD96 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD96 (CD96) (Cys110Ser-Mutant) protein (His tag)CD96 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD96 (CD96) (AA 22-536) protein (His tag)CD96 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD96 (CD96) (AA 22-503) protein (mFc-His Tag)CD96 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
CD96 (CD96) (AA 22-503) protein (His-Avi Tag,Biotin)CD96 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD96 (CD96) (AA 1-402) protein (GST tag)CD96 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
CD96 (CD96) (Active) protein (His tag)CD96 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
CD96 (CD96) (AA 22-503) (Active) protein (Fc Tag)CD96 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by reduced SDS-PAGE. Active
CD96 (CD96) (AA 22-503) (Active) protein (His tag)CD96 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CD96
- Alternative Name
- CD96 (CD96 Products)
- Background
- The receptors CD96 and TIGIT are expressed on the surface of T and natural killer (NK) cells, and recent studies suggest both play important inhibitory roles in immune function. CD96 has been shown to modulate immune cell activity in mice, with Cd96-/- mice displaying hypersensitive NK-cell responses to immune challenge and significant tumor resistance. The counterbalance between the putative inhibitory CD96 and TIGIT receptors and the activating receptor, CD226, offers unique strategies for immuno-oncology drug development.
- Molecular Weight
- 54.7 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.
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