CTGF Protein (AA 27-349) (His-Avi Tag,Biotin)

Catalog No. ABIN7274343

Product Details

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Protein Type: Recombinant

Protein Characteristics: AA 27-349

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CTGF protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human CTGF/CCN2 Protein

Sequence: Gln27-Ala349

Characteristics: Recombinant Biotinylated Human CTGF/CCN2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln27-Ala349.

Purity: > 95 % as determined by Tris-Bis PAGE

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-CTGF Antibody at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CTGF, His Tag with the EC50 of 32.6ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS, 200 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Alternative Name: CTGF (CTGF Products)

Background: Connective tissue growth factor (CTGF) is a member of the CCN matricellular protein family, consisting of four domains, that regulates the signaling of other growth factors and promotes kidney fibrosis.CTGF can simultaneously interact with several factors with its four domains. The microenvironment differs depending on the types of cells and tissues and differentiation stages of these cells.

Molecular Weight: 38.4 kDa. Due to glycosylation, the protein migrates to 43-53 kDa based on Tris-Bis PAGE result.

Pathways: Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Growth Factor Binding