CLEC9A Protein (AA 57-241) (Fc Tag)
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- Target See all CLEC9A Proteins
- CLEC9A (C-Type Lectin Domain Family 9, Member A (CLEC9A))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 57-241
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CLEC9A protein is labelled with Fc Tag.
- Purpose
- Human CLEC9A Protein
- Sequence
- Lys57-Val241
- Characteristics
- Recombinant Human CLEC9A Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Lys57-Val241.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CLEC9A, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CLEC9A Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
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C-Type Lectin Domain Family 9, Member A (CLEC9A) (AA 57-264) protein (Fc Tag)
CLEC9A Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
C-Type Lectin Domain Family 9, Member A (CLEC9A) (AA 100-209) protein (His tag)CLEC9A Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
C-Type Lectin Domain Family 9, Member A (CLEC9A) (AA 1-241) protein (GST tag)CLEC9A Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CLEC9A (C-Type Lectin Domain Family 9, Member A (CLEC9A))
- Alternative Name
- CLEC9A (CLEC9A Products)
- Synonyms
- 9830005G06Rik Protein, DNGR-1 Protein, DNGR1 Protein, UNQ9341 Protein, RGD1562513 Protein, C-type lectin domain family 9, member a Protein, C-type lectin domain containing 9A Protein, C-type lectin domain family 9, member A Protein, Clec9a Protein, CLEC9A Protein
- Background
- CLEC9A expression was significantly higher in psoriatic skin compared with healthy donor. In psoriatic skin and PsA ST, CLEC9A() cells were in close proximity to TUNEL() cells. SF CLEC9A levels were significantly lower compared with paired PsA serum. Adalimumab treatment did not affect CLEC9A serum level and skin expression. The downregulation of synovial CLEC9A might be associated with a novel mechanism by which anti-TNF therapy might reduce CD8-mediated inflammation in PsA patients.
- Molecular Weight
- 48.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
- UniProt
- Q6UXN8
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