Clusterin Protein (CLU) (AA 23-449) (His tag)
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- Target See all Clusterin (CLU) Proteins
- Clusterin (CLU)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 23-449
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Clusterin protein is labelled with His tag.
- Purpose
- Human Clusterin Protein
- Sequence
- Asp23-Glu449
- Characteristics
- Recombinant Human Clusterin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp23-Glu449.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human Clusterin, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Clusterin Antibody, hFc Tag with the EC50 of 63.4ng/ml determined by ELISA. See testing image for detail.
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Clusterin (CLU) (AA 22-448) protein (His tag)
CLU Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Clusterin (CLU) (AA 184-293) protein (His-IF2DI Tag)CLU Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Clusterin (CLU) (AA 402-501) protein (GST tag)CLU Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Clusterin (CLU)
- Alternative Name
- Clusterin (CLU Products)
- Background
- Clusterin (CLU) is a stress-activated, ATP-independent molecular chaperone, normally secreted from cells, that is up-regulated in Alzheimer disease and in many cancers. It plays important roles in protein homeostasis/proteostasis, inhibition of cell death pathways, and modulation of pro-survival signalling and transcriptional networks. Changes in the CLU gene locus are highly associated with Alzheimer disease, and many therapy-resistant cancers over-express CLU.
- Molecular Weight
- 51.2 kDa. The protein migrates to 38-41 kDa based on Tris-Bis PAGE result.
- Pathways
- Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
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