FBLN3 Protein (AA 18-493) (His tag)
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- Target See all FBLN3 Proteins
- FBLN3 (Fibulin 3 (FBLN3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 18-493
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This FBLN3 protein is labelled with His tag.
- Purpose
- Human EFEMP1 Protein
- Sequence
- Gln18-Phe493
- Characteristics
- Recombinant Human EFEMP1 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln18-Phe493.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human EFEMP1, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-EFEMP1 Antibody, hFc Tag with the EC50 of 35.1ng/ml determined by ELISA. See testing image for detail.
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Fibulin 3 (FBLN3) (AA 1-493) protein (GST tag)
FBLN3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Fibulin 3 (FBLN3) (Transcript Variant 1) (Active) protein (Myc-DYKDDDDK Tag)FBLN3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD, Func, PI Active
Fibulin 3 (FBLN3) (AA 18-493) protein (His tag)FBLN3 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Fibulin 3 (FBLN3) (AA 18-493) protein (His tag)Crystallography grade FBLN3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Fibulin 3 (FBLN3) (AA 18-493) protein (His tag)Crystallography grade FBLN3 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Fibulin 3 (FBLN3) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)FBLN3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- FBLN3 (Fibulin 3 (FBLN3))
- Alternative Name
- EFEMP1 (FBLN3 Products)
- Synonyms
- DHRD Protein, DRAD Protein, FBLN3 Protein, FBNL Protein, FIBL-3 Protein, MLVT Protein, MTLV Protein, S1-5 Protein, T16 Protein, EGF containing fibulin like extracellular matrix protein 1 Protein, EGF-containing fibulin-like extracellular matrix protein 1 Protein, epidermal growth factor-containing fibulin-like extracellular matrix protein 1 Protein, EFEMP1 Protein, Efemp1 Protein
- Background
- Transversalis fascia EFEMP1, TIMP3 and ELN expressions were decreased, but MMP9 expression was increased in IH patients compared with controls. In IH patients, EFEMP1 was not correlated with TIMP3, but positively correlated with ELN and negatively correlated with MMP9, TIMP3 negatively correlated with MMP9, but positively correlated with ELN.In addition, EFEMP1 suppressed L929 cell migration and invasion.
- Molecular Weight
- 53.9 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
- Pathways
- EGFR Signaling Pathway
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