Fc gamma RII (CD32) (AA 17-208) protein (His tag)
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- Target See all Fc gamma RII (CD32) Proteins
- Fc gamma RII (CD32)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 17-208
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Origin
- Cynomolgus
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- His tag
- Purpose
- Cynomolgus Fc gamma RIII/CD16 Protein
- Sequence
- Gly17-Gln208
- Characteristics
- Recombinant Cynomolgus Fc gamma RIII/CD16 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly17-Gln208.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- The affinity constant of 0.251 μM as determined in SPR assay (Biacore T200). See testing image for detail.
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Fc gamma RII (CD32) proteinCD32 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % ELISA, WB, SDS, IP
Fc gamma RII (CD32) proteinCD32 Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % ELISA, WB, SDS, IP
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Fc gamma RII (CD32)
- Alternative Name
- Fc gamma RIII (CD32 Products)
- Background
- Immunoglobulin G (IgG) Fc receptors play a critical role in linking IgG antibody-mediated immune responses with cellular effector functions. A high resolution map of the binding site on human IgG1 for human Fc gamma RI, Fc gamma RIIA, Fc gamma RIIB, Fc gamma RIIIA, and FcRn receptors has been determined.A common set of IgG1 residues is involved in binding to all Fc gamma R, Fc gamma RII and Fc gamma RIII also utilize residues outside this common set.
- Molecular Weight
- 23.1 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Tris-Bis PAGE result.
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