Interferon gamma Protein (IFNG) (AA 24-161) (His-Avi Tag,Biotin)

Catalog No. ABIN7274848

Product Details

Target: Interferon gamma (IFNG)

Protein Type: Recombinant

Protein Characteristics: AA 24-161

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Interferon gamma protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human IFN gamma/IFNG Protein

Sequence: Gln24-Gly161

Characteristics: Recombinant Biotinylated Human IFN gamma/IFNG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Gly161.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IFNGR1, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IFN gamma, His Tag with the EC50 of 39.1g/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Interferon gamma (IFNG)

Alternative Name: IFN gamma (IFNG Products)

Background: Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease.

Molecular Weight: 19.1 kDa. Due to glycosylation, the protein migrates to 25-40 kDa based on Tris-Bis PAGE result.

UniProt: P01579

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy