IgG2a Fc (AA 98-330) Protein
Origin: Mouse
Host: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
-
- Target
- IgG2a Fc
- Protein Type
- Recombinant
- Protein Characteristics
- AA 98-330
-
Origin
- Mouse
-
Source
- HEK-293 Cells
- Purpose
- Mouse IgG2a Fc Protein
- Sequence
- Glu98-Lys330
- Characteristics
- Recombinant Mouse IgG2a Fc Protein is expressed from HEK293 without tag.It contains Glu98-Lys330.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- The affinity constant of 2.72 nM as determined in SPR assay (Biacore T200). See testing image for detail.
-
IgG2a Fc protein (Fc Tag)
Origin: Rat Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- IgG2a Fc
- Background
- Mouse IgG2a is one of the subtypes of murine immunoglobulin IgG which mediates immune responses to carbohydrate or polysaccharide antigens. The Fc region of IgG2a can bind to FcRn to prolong its half-life.
- Molecular Weight
- 26.38 kDa. Due to glycosylation, the protein migrates to 33-38 kDa based on Tris-Bis PAGE result.
- UniProt
- P01863
-
You are here:
