IL1RL2 Protein (Cys154Ser-Mutant, Cys262Ser-Mutant) (His tag)

Catalog No. ABIN7274886

Product Details

Target: IL1RL2 (Interleukin 1 Receptor-Like 2 (IL1RL2))

Protein Type: Recombinant

Protein Characteristics: Cys154Ser-Mutant, Cys262Ser-Mutant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IL1RL2 protein is labelled with His tag.

Purpose: Human IL-1 Rrp2/IL-1 R6 (C154S,C262S) Protein

Sequence: Asp20-Tyr337 (C154S,C262S)

Characteristics: Recombinant Human IL-1 Rrp2/IL-1 R6 (C154S,C262S) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp20-Tyr337 (C154S,C262S).

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-1 Rrp2 (C154S,C262S) , His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IL-1 Rrp-2 Antibody, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL1RL2 (Interleukin 1 Receptor-Like 2 (IL1RL2))

Alternative Name: IL-1 Rrp2 (IL1RL2 Products)

Background: The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL-1 R3), ST2 (T1/IL-1 R4), IL-18 Ra (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 Rb (AcPL/IL-1 R7), IL-1RAPL-1 (TIGIRR-2/IL-1 R8), and TIGIRR-1 (IL-1 R9). IL1RL2 is a receptor for interleukin-36 (IL36A, IL36B and IL36G). After binding to interleukin-36 associates with the coreceptor IL1RAP to form the interleukin-36 receptor complex which mediates interleukin-36-dependent activation of NF-kappa-B, MAPK and other pathways.

Molecular Weight: 37.2 kDa. Due to glycosylation, the protein migrates to 56-67 kDa based on Tris-Bis PAGE result.