Interleukin 17a Protein (AA 24-155) (His tag)
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- Target See all Interleukin 17a (IL17A) Proteins
- Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 24-155
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Interleukin 17a protein is labelled with His tag.
- Purpose
- Human IL-17A/CTLA-8 Protein
- Sequence
- Gly24-Ala155
- Characteristics
- Recombinant Human IL-17A/CTLA-8 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly24-Ala155.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-17A, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 78.0ng/ml determined by ELISA. The affinity constant of 1.57nM as determined in SPR assay (Biacore T200). See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
- Alternative Name
- IL-17A (IL17A Products)
- Background
- Interleukin-17A (IL-17A), also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.
- Molecular Weight
- 18.2 kDa. Due to glycosylation, the protein migrates to 16-30 kDa based on Tris-Bis PAGE result.
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