Integrin alpha 5 beta 1 (ITGA5 & ITGB1) Heterodimer (AA 42-995) protein (His-Avi Tag,Biotin)
Origin: Human
Host: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
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- Target
- Integrin alpha 5 beta 1 (ITGA5 & ITGB1) Heterodimer
- Protein Type
- Recombinant
- Protein Characteristics
- AA 42-995
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- His-Avi Tag,Biotin
- Purpose
- Biotinylated Human Integrin alpha 5 beta 1 (ITGA5 & ITGB1) Heterodimer Protein
- Sequence
- Phe42-Tyr995 (ITGA5) acidic tail & Gln21-Asp728 (ITGB1) basic tail
- Specificity
- Uni-Prot: P08648 (ITGA5), P05556-1 (ITGB1)
- Characteristics
- Recombinant Biotinylated Human Integrin alpha 5 beta 1 (ITGA5 & ITGB1) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe42-Tyr995(ITGA5) acidic tail & Gln21-Asp728(ITGB1) basic tail.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized 0.5μg Anti-ITGA5&ITGB1 Antibody, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human ITGA5&ITGB1, His Tag with the EC50 of 55.3ng/ml determined by ELISA. See testing image for detail.
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Integrin alpha 5 beta 1 (ITGA5 & ITGB1) Heterodimer (AA 42-995) protein (His-Avi Tag)
Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Integrin alpha 5 beta 1 (ITGA5 & ITGB1) Heterodimer
- Background
- Integrin alpha 5/ beta 1, also known as VLA-5, is a widely expressed non-covalent heterodimer of a 160 kDa alpha 5 and a 130 kDa beta 1 Integrin subunit.Alpha 5/ beta 1 is up-regulated on tumor vasculature and promotes angiogenesis.
- Molecular Weight
- 111.8 kDa (ITGA5)&83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 110-140 kDa based on Tris-Bis PAGE result.
- UniProt
- P08648
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