Integrin alpha A1 beta 1 (ITGA1 & ITGB1) Heterodimer (AA 29-1141) protein (His tag)

Catalog No. ABIN7277143

Product Details

Target: Integrin alpha A1 beta 1 (ITGA1 & ITGB1) Heterodimer

Protein Type: Recombinant

Protein Characteristics: AA 29-1141

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: His tag

Purpose: Mouse Integrin alpha 1 beta 1 (ITGA1 & ITGB1) Heterodimer Protein

Sequence: Phe29-Pro1141 (ITGA1) acidic tail & Gln21-Asp728 (ITGB1) basic tail

Specificity: Uni-Prot: Q3V3R4 (ITGA1), P09055-1 (ITGB1)

Characteristics: Recombinant Mouse Integrin alpha 1 beta 1 (ITGA1 & ITGB1) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe29-Pro1141(ITGA1) acidic tail & Gln21-Asp728(ITGB1) basic tail.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Integrin alpha A1 beta 1 (ITGA1 & ITGB1) Heterodimer

Background: Integrin α1β1 is a heterodimer of the Integrin subunits alpha 1 and beta 1 and is one of four collagen-binding integrins, it preferentially binds Collagens I, IV, VI, XIII and XVI, but also binds Laminin. Integrin α1β1 is widely expressed in mesenchyme and the immune system, as well as a minority of epithelial tissues. It can suppresse EGFR signaling, increase expression of Caveolin-1, reduce production of reactive oxygen species, regulate collagen expression, regulate MMP collagenase and gelatinase activity, and mediates the renal basement membrane disorder Alport syndrome.

Molecular Weight: 129.00 kDa (ITGA1)&82.80 kDa (ITGB1). Due to glycosylation, the protein migrates to 140-200 kDa (ITGA1) and 100-115 kDa (ITGB1) based on Tris-Bis PAGE result.

UniProt: Q3V3R4