Integrin alpha 2 beta 1 (ITGA2 & ITGB1) Heterodimer (AA 27-1129) protein (His tag)
Origin: Mouse
Host: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
-
- Target
- Integrin alpha 2 beta 1 (ITGA2 & ITGB1) Heterodimer
- Protein Type
- Recombinant
- Protein Characteristics
- AA 27-1129
- Origin
- Mouse
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- His tag
- Purpose
- Mouse Integrin alpha 2 beta 1 (ITGA2 & ITGB1) Heterodimer Protein
- Sequence
- Tyr27-Thr1129 (ITGA2) acidic tail & Gln21-Asp728 (ITGB1) basic tail
- Specificity
- Uni-Prot: Q62469 (ITGA2), P09055-1 (ITGB1)
- Characteristics
- Recombinant Mouse Integrin alpha 2 beta 1(ITGA2 & ITGB1) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr27-Thr1129(ITGA2) acidic tail & Gln21-Asp728(ITGB1) basic tail.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
-
Integrin alpha 2 beta 1 (ITGA2 & ITGB1) Heterodimer (AA 30-1132) protein (His tag)
Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- Integrin alpha 2 beta 1 (ITGA2 & ITGB1) Heterodimer
- Background
- Integrin alpha 2 beta 1 is a positive regulator of collagenase (MMP-1) and collagen alpha 1(I) gene expression.HOS cells normally lacking alpha 2 beta 1 integrin were forced to express it, and this prevented the down-regulation in the levels of alpha 1 (I) collagen mRNA when cells were grown inside collagen gels. The data indicate that the level of MMP-1 expression is regulated by the collagen receptor alpha 2 beta 1 integrin.
- Molecular Weight
- 126.60 kDa(ITGA2)&82.80 kDa(ITGB1). Due to glycosylation, the protein migrates to 128-138 kDa(ITGA2)&105-115 kDa(ITGB1) based on Tris-Bis PAGE result.
- UniProt
- Q62469
-
You are here:
