MFI2 Protein (AA 20-709) (His tag)
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- Target See all MFI2 Proteins
- MFI2 (Antigen P97 (Melanoma Associated) Identified By Monoclonal Antibodies 133.2 and 96.5 (MFI2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 20-709
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This MFI2 protein is labelled with His tag.
- Purpose
- Human CD228/MFI2 Protein
- Sequence
- Gly20-Cys709
- Characteristics
- Recombinant Human CD228/MFI2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Cys709.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Antigen P97 (Melanoma Associated) Identified By Monoclonal Antibodies 133.2 and 96.5 (MFI2) (AA 20-709) protein (His tag)
MFI2 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Antigen P97 (Melanoma Associated) Identified By Monoclonal Antibodies 133.2 and 96.5 (MFI2) (AA 20-709) protein (His tag)MFI2 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Antigen P97 (Melanoma Associated) Identified By Monoclonal Antibodies 133.2 and 96.5 (MFI2) (AA 20-708) protein (His tag)MFI2 Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Antigen P97 (Melanoma Associated) Identified By Monoclonal Antibodies 133.2 and 96.5 (MFI2) (AA 21-302) protein (GST tag)MFI2 Origin: Human Host: Wheat germ Recombinant WB, AP, AA, ELISA
Antigen P97 (Melanoma Associated) Identified By Monoclonal Antibodies 133.2 and 96.5 (MFI2) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)MFI2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Antigen P97 (Melanoma Associated) Identified By Monoclonal Antibodies 133.2 and 96.5 (MFI2) (AA 20-710), (partial) protein (His-SUMO Tag)MFI2 Origin: Rabbit Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- MFI2 (Antigen P97 (Melanoma Associated) Identified By Monoclonal Antibodies 133.2 and 96.5 (MFI2))
- Alternative Name
- CD228 (MFI2 Products)
- Background
- Melanotransferrin is typically overexpressed in melanoma cells compared to other cell types - including cancer cells - and is efficiently sorted and secreted with nanovesicles, or so-called exosomes, due to its membrane-anchoring by a glycosylphosphatidylinositol. Melanotransferrin is exposed on the surface of exosomes and is accessible for antibody recognition.
- Molecular Weight
- 76.60 kDa. Due to glycosylation, the protein migrates to 78-88 kDa based on Tris-Bis PAGE result.
- Pathways
- Transition Metal Ion Homeostasis, Cell-Cell Junction Organization
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