NKG2A & CD94 (AA 100-219) protein (Fc-DYKDDDDK Tag)

Catalog No. ABIN7275325

Product Details

Target: NKG2A & CD94

Protein Type: Recombinant

Protein Characteristics: AA 100-219

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: Fc-DYKDDDDK Tag

Purpose: Human NKG2A & CD94 Protein

Sequence: Arg100-Ala219 (NKG2A) & Ser34-Ile179 (CD94)

Specificity: Uni-Prot: P26715-1 (NKG2A), Q13241-1 (CD94)

Characteristics: Recombinant Human NKG2A & CD94 Protein is expressed from HEK293 with hFc tag and Flag tag at the N-Terminus.It contains Arg100-Ala219(NKG2A) & Ser34-Ile179(CD94).

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human NKG2A&CD94, hFc tag at 1μg/ml (100μl/Well) on Fc Antibody (5μg/ml) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Ab., hFc Tag with the EC50 of 15.1ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: NKG2A & CD94

Alternative Name: NKG2A&CD94

Background: The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.

Molecular Weight: 17.9 kDa (CD94)&41.1 kDa (NKG2A). Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.