NKG2A & CD94 (AA 100-233) protein (His-Avi Tag,Biotin)
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- Target
- NKG2A & CD94
- Protein Type
- Recombinant
- Protein Characteristics
- AA 100-233
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- His-Avi Tag,Biotin
- Purpose
- Biotinylated Human NKG2A & CD94 Protein
- Sequence
- Arg100-Leu233 (NKG2A) & Ser34-Ile179 (CD94)
- Specificity
- Uni-Prot: P26715-1 (NKG2A), Q13241-1 (CD94)
- Characteristics
- Recombinant Biotinylated Human NKG2A & CD94 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94).
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Anti-NKG2A Antibody, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human NKG2A&CD94, His Tag with the EC50 of 0.11μg/ml determined by ELISA. See testing image for detail.
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NKG2A & CD94 protein (mFc Tag)
Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
NKG2A & CD94 protein (His-Avi Tag)Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
NKG2A & CD94 (AA 100-233) protein (Fc-DYKDDDDK Tag)Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
NKG2A & CD94 (AA 100-219) protein (Fc-DYKDDDDK Tag)Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
NKG2A & CD94 (AA 98-244) protein (mFc Tag)Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
NKG2A & CD94 (AA 94-233) protein (His-DYKDDDDK Tag)Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
NKG2A & CD94 (AA 113-233) protein (Fc Tag)Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
NKG2A & CD94 (AA 113-233), (AA 57-179) (Active) protein (Fc Tag,AVI tag,Biotin)Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- NKG2A & CD94
- Alternative Name
- NKG2A&CD94
- Background
- The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.
- Molecular Weight
- 17.9 kDa (CD94)&18.3 kDa (NKG2A). Due to glycosylation, the protein migrates to 33-48 kDa based on Tris-Bis PAGE result.
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