Periostin Protein (POSTN) (AA 22-836) (His tag)
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- Target See all Periostin (POSTN) Proteins
- Periostin (POSTN)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-836
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Periostin protein is labelled with His tag.
- Purpose
- Human Periostin/OSF-2 Protein
- Sequence
- Asn22-Gln836
- Characteristics
- Recombinant Human Periostin/OSF-2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asn22-Gln836.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human Periostin, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Periostin Antibody, hFc Tag with the EC50 of 75.7ng/ml determined by ELISA. See testing image for detail.
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Periostin (POSTN) (AA 24-811) protein (His tag)
POSTN Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Periostin (POSTN) (Active) protein (His tag)POSTN Origin: Mouse Host: HEK-293 Cells Recombinant > 92 % as determined by SDS-PAGE Active
Periostin (POSTN) (Active) protein (His tag)POSTN Origin: Human Host: HEK-293 Cells Recombinant > 97 % as determined by reducing SDS-PAGE. Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Periostin (POSTN)
- Alternative Name
- Periostin (POSTN Products)
- Background
- Periostin is a matricellular protein that is expressed in several tissues during embryonic development, however, its expression in adults is mostly restricted to collagen-rich connective tissues. Periostin is expressed only briefly during kidney development, but it is not normally detected in the adult kidney. Recent evidence has revealed that periostin is aberrantly expressed in several forms of chronic kidney disease (CKD), and that its expression correlates with the degree of interstitial fibrosis and the decline in renal function.
- Molecular Weight
- 92.1 kDa. Due to glycosylation, the protein migrates to 93-100 kDa based on Tris-Bis PAGE result.
- UniProt
- Q15063
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