Phospholipase D4 Protein (PLD4) (AA 52-506) (His tag)
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- Target See all Phospholipase D4 (PLD4) Proteins
- Phospholipase D4 (PLD4)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 52-506
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Phospholipase D4 protein is labelled with His tag.
- Purpose
- Human PLD4 Protein
- Sequence
- Trp52-Gly506
- Characteristics
- Recombinant Human PLD4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp52-Gly506.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Top Product
- Discover our top product PLD4 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 50 mM MES, 100 mM NaCl ( pH 6.0).
- Buffer
- Lyophilized from 0.22μm filtered solution in 50 mM MES, 100 mM NaCl ( pH 6.0). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Phospholipase D4 (PLD4)
- Alternative Name
- PLD4 (PLD4 Products)
- Background
- Phospholipase D4 (PLD4) is a newly identified protein expressed in microglia. the expression of PLD4 was located in macrophages in the colon cancer mesenchymal and lymph nodes as shown by immunohistochemical analysis. furthermore, its expression was associated with clinical staging of colon cancer. Then, THP-1 as a cell model induced into TAMs. PLD4 could be involved in the activation process of M1 phenotype macrophages.
- Molecular Weight
- 51.1 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result.
- UniProt
- Q96BZ4
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