SPARC Protein (AA 18-303) (His tag)
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- Target See all SPARC Proteins
- SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 18-303
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SPARC protein is labelled with His tag.
- Purpose
- Human SPARC Protein
- Sequence
- Ala18-Ile303
- Characteristics
- Recombinant Human SPARC Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala18-Ile303.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human SPARC, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 66.8ng/ml determined by ELISA. See testing image for detail.
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Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) protein (His tag)
SPARC Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-302) protein (His tag)SPARC Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) protein (Fc Tag)SPARC Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-297) protein (His tag)SPARC Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 1-303) protein (GST tag)SPARC Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303), (full length) protein (GST tag)SPARC Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))
- Alternative Name
- SPARC (SPARC Products)
- Background
- Secreted protein acidic and rich in cysteine (SPARC/osteonectin/BM40) is one of the most abundant non-collagenous protein expressed in mineralized tissues.The capacity of SPARC to influence pathways involved in extracellular matrix assembly such as procollagen processing and collagen fibril formation as well as the capacity to influence osteoblast differentiation and osteoclast activity will be addressed.
- Molecular Weight
- 33.8 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
- UniProt
- P09486
- Pathways
- Autophagy
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