Osteopontin Protein (AA 17-300) (His tag)
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- Target See all Osteopontin (SPP1) Proteins
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 17-300
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Osteopontin protein is labelled with His tag.
- Purpose
- Human SPP1/OPN Protein
- Sequence
- Ile17-Asn300
- Characteristics
- Recombinant Human SPP1/OPN Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile17-Asn300.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Secreted phosphoprotein 1 (SPP1) (AA 17-294) protein (His tag)
SPP1 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Secreted phosphoprotein 1 (SPP1) (AA 28-61) protein (His-IF2DI Tag)SPP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Secreted phosphoprotein 1 (SPP1) (AA 1-300) protein (GST tag)SPP1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Secreted phosphoprotein 1 (SPP1) proteinSPP1 Origin: Human Native > 90 % (SDS-PAGE)
Secreted phosphoprotein 1 (SPP1) (AA 17-300), (partial) protein (His tag)SPP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
Secreted phosphoprotein 1 (SPP1) (AA 17-287) (Active) protein (His tag)SPP1 Origin: Human Host: Escherichia coli (E. coli) > 95 % AcA, CC Active
Secreted phosphoprotein 1 (SPP1) (AA 17-317) (Active) protein (His tag)SPP1 Origin: Rat Host: HEK-293 Cells > 95 % AcA, CC Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
- Alternative Name
- SPP1 (SPP1 Products)
- Background
- Ovarian cancer is one of the most lethal malignant tumors in women. Secreted phosphoprotein 1 (SPP1) plays an important role in some cancer types. The expression of SPP1 was higher in epithelial ovarian cancer tissues than in normal ovarian tissues. Silencing SPP1 decreased the cell proliferation, migration, and invasion. Ectopic expression of SPP1 increased the cell proliferation, migration, and invasion. Silencing SPP1 prevented ovarian cancer growth in mice. Silencing SPP1 inhibited Integrin β1/FAK/AKT pathway.
- Molecular Weight
- 34.8 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
- NCBI Accession
- NP_000573
- Pathways
- Regulation of Cell Size
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