Syndecan 1 Protein (SDC1) (AA 23-254) (His tag)

Catalog No. ABIN7275677

Product Details

Target: Syndecan 1 (SDC1)

Protein Type: Recombinant

Protein Characteristics: AA 23-254

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Syndecan 1 protein is labelled with His tag.

Purpose: Human Syndecan-1 Protein

Sequence: Gln23-Gly254

Characteristics: Recombinant Human Syndecan-1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln23-Gly254.

Purity: > 95 % as determined by Tris-Bis PAGE

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Syndecan-1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Syndecan-1 Antibody, hFc Tag with the EC50 of 28.2ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Syndecan 1 (SDC1)

Alternative Name: Syndecan-1 (SDC1 Products)

Background: Liver diseases such as liver cirrhosis, primary and metastatic liver cancers are still a major medical challenge. Syndecan-1 is one of the most important proteoglycans in the liver. Syndecan-1 is normally expressed on the surfaces of hepatocytes and cholangiocytes. Due to liver diseases the amount of syndecan-1 increases in the liver.

Molecular Weight: 25 kDa. Due to glycosylation, the protein migrates to 50-80 kDa based on Tris-Bis PAGE result.

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism