CD248 Protein (AA 18-687) (His tag)

Catalog No. ABIN7275687

Product Details

Target: CD248 (CD248 Molecule, Endosialin (CD248))

Protein Type: Recombinant

Protein Characteristics: AA 18-687

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CD248 protein is labelled with His tag.

Purpose: Human TEM1/cd248 Protein

Sequence: Gln18-Leu687

Characteristics: Recombinant Human TEM1/cd248 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln18-Leu687.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human TEM1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TEM1 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD248 (CD248 Molecule, Endosialin (CD248))

Alternative Name: TEM1 (CD248 Products)

Background: Colorectal cancer (CRC) is one of the most common cancers worldwide usually diagnosed in the advanced stage. The serum concentration of tumor endothelial marker 1 (TEM1) was measured and correlated with clinicopathological features to evaluate whether TEM1 might serve as a biomarker for early CRC diagnosis, progression, and prognosis. TEM1 can act as a potential diagnostic, progression, and prognostic serum biomarker for patients with CRC, TEM1 might be a good supplement for commonly used markers CEA and Ca 19-9.

Molecular Weight: 72.7 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.