Latent TGF-beta 1 protein (His tag)

Catalog No. ABIN7275704

Product Details

Target: Latent TGF-beta 1

Protein Type: Recombinant

Origin: Rhesus Monkey

Source: HEK-293 Cells

Purification tag / Conjugate: His tag

Sequence: Leu30-Ser390(C33S)

Purity: > 95% as determined by Tris-Bis PAGE

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Rhesus macaque Latent TGF beta 1, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, His Tag with the EC50 of 1.2μg/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Storage: 4 °C,-80 °C

Storage Comment: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Latent TGF-beta 1

Alternative Name: Latent TGF beta 1

Background: CEDLAP, DPD1, TGF beta1, TGFB, TGFB1, TGFbeta, TGF-beta-1,Latent TGF beta 1  cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 1. Disulfide-linked homodimers of LAP and TGF- beta 1 remain non-covalently associated after secretion, forming the small latent TGF- beta 1 complex.

Molecular Weight: 31.4/12.8 kDa. Due to glycosylation, the protein migrates to 40-50/14-15 kDa based on Tris-Bis PAGE result.

UniProt: F7HCV5