Latent TGF-beta 1 (AA 30-390) protein (His-Avi Tag,Biotin)
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- Target
- Latent TGF-beta 1
- Protein Type
- Recombinant
- Protein Characteristics
- AA 30-390
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- His-Avi Tag,Biotin
- Purpose
- Biotinylated Human Latent TGF beta 1/TGFB1 Protein
- Sequence
- Leu30-Ser390 (C33S)
- Characteristics
- Recombinant Biotinylated Human Latent TGF beta 1/TGFB1 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Leu30-Ser390(C33S).
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 93 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human ITGAV&ITGB6, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 65.7ng/ml determined by ELISA. See testing image for detail.
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Latent TGF-beta 1 (AA 30-390) protein (His tag)
Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Latent TGF-beta 1 protein (His tag)Origin: Rhesus Monkey Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE
Latent TGF-beta 1 (AA 30-390) (Active) protein (His tag)Origin: Cynomolgus Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Latent TGF-beta 1 protein (His tag)Origin: Mouse Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Latent TGF-beta 1
- Alternative Name
- Latent TGF beta 1
- Background
- Latent TGF beta 1 cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 1. Disulfide-linked homodimers of LAP and TGF- beta 1 remain non-covalently associated after secretion, forming the small latent TGF- beta 1 complex.
- Molecular Weight
- 44.2 kDa. Due to glycosylation, the protein migrates to 40-50 (LAP) /13-15 (Mature TGF beta 1) kDa based on Tris-Bis PAGE result.
- UniProt
- P01137
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