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Latent TGF-beta 1 protein (His tag)

Origin: Mouse Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE
Catalog No. ABIN7275709
  • Target
    Latent TGF-beta 1
    Protein Type
    Recombinant
    Origin
    • 2
    • 1
    • 1
    • 1
    Mouse
    Source
    • 5
    HEK-293 Cells
    Purification tag / Conjugate
    His tag
    Sequence
    Leu30-Ser390
    Purity
    > 95% as determined by Tris-Bis PAGE
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1EU per μg by the LAL method.
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
    Storage
    4 °C,-80 °C
    Storage Comment
    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    Latent TGF-beta 1
    Alternative Name
    Latent TGF beta 1
    Background
    CEDLAP, DPD1, TGF beta1, TGFB, TGFB1, TGFbeta, TGF-beta-1,Latent TGF beta 1  cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 1. Disulfide-linked homodimers of LAP and TGF- beta 1 remain non-covalently associated after secretion, forming the small latent TGF- beta 1 complex.
    Molecular Weight
    29.5/12.9 kDa. Due to glycosylation, the protein migrates to 40-50/14-15 kDa based on Tris-Bis PAGE result.
    UniProt
    P04202
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