TNFRSF1A Protein (AA 30-211) (His tag)
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- Target See all TNFRSF1A Proteins
- TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 30-211
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This TNFRSF1A protein is labelled with His tag.
- Purpose
- Human TNFR1/CD120a/TNFRSF1A Protein
- Sequence
- Leu30-Thr211
- Characteristics
- Recombinant Human TNFR1/CD120a/TNFRSF1A Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu30-Thr211.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human TNFR1, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 4.8ng/ml determined by ELISA. See testing image for detail.
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Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) (AA 30-211) protein (Fc Tag)
TNFRSF1A Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) (AA 30-211) protein (His tag)TNFRSF1A Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) (AA 22-211) protein (His-DYKDDDDK-Strep II Tag)TNFRSF1A Origin: Human Host: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) proteinTNFRSF1A Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE
Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) (AA 22-212) protein (His tag)TNFRSF1A Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) (AA 22-211) protein (His tag)TNFRSF1A Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % by SDS - PAGE SDS
Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) (AA 31-130) protein (GST tag)TNFRSF1A Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) (Active) protein (His tag)TNFRSF1A Origin: Mouse Host: HEK-293 Cells Recombinant > 96 % as determined by SDS-PAGE Active
Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) (AA 1-455) protein (GST tag)TNFRSF1A Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) (Active) protein (Fc Tag)TNFRSF1A Origin: Rat Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))
- Alternative Name
- TNFR1 (TNFRSF1A Products)
- Background
- Tumour necrosis factor alpha (TNF-α) is a pleiotropic cytokine with both injurious and protective functions, which are thought to diverge at the level of its two cell surface receptors, TNFR1 and TNFR2. In the setting of acute injury, selective inhibition of TNFR1 is predicted to attenuate the cell death and inflammation associated with TNF-α, while sparing or potentiating the protective effects of TNFR2 signalling.
- Molecular Weight
- 23.3 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result.
- Pathways
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Hepatitis C, Ubiquitin Proteasome Pathway
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