WISP1 Protein (AA 23-367) (His tag)
-
- Target See all WISP1 Proteins
- WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 23-367
-
Origin
- Mouse
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This WISP1 protein is labelled with His tag.
- Purpose
- Mouse WISP1 Protein
- Sequence
- Thr23-Asn367
- Characteristics
- Recombinant Mouse WISP1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr23-Asn367.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
-
WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (AA 1-280) protein (GST tag)
WISP1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (Transcript Variant 1) (Active) proteinWISP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD, Func, PI Active
WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)WISP1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
- Alternative Name
- WISP1 (WISP1 Products)
- Background
- The interplay between glioma stem cells (GSCs) and the tumor microenvironment plays crucial roles in promoting malignant growth of glioblastoma (GBM), the most lethal brain tumor. WISP1 is preferentially expressed and secreted by GSCs. Silencing WISP1 markedly disrupts GSC maintenance, reduces tumor-supportive TAMs (M2), and potently inhibits GBM growth. WISP1 signals through Integrin α6β1-Akt to maintain GSCs by an autocrine mechanism and M2 TAMs through a paracrine manner.
- Molecular Weight
- 39.4 kDa. Due to glycosylation, the protein migrates to 64-68 kDa based on Tris-Bis PAGE result.
- UniProt
- O54775
- Pathways
- WNT Signaling, Growth Factor Binding
-
