Nucleolin Protein (NCL) (AA 2-710) (His tag)
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- Target See all Nucleolin (NCL) Proteins
- Nucleolin (NCL)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-710
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Nucleolin protein is labelled with His tag.
- Purpose
- Recombinant human NCL protein with N-terminal 6xHis tag
- Specificity
- 6xHis tag NCL (Val2-Glu710)
- Characteristics
- Extracellular Domain Protein
- Purification
- Purified from cell culture supernatant by affinity chromatography
- Purity
- The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
- Top Product
- Discover our top product NCL Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- Expiry Date
- 12 months
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- Target
- Nucleolin (NCL)
- Alternative Name
- NCL (NCL Products)
- Background
- Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]
- Molecular Weight
- predicted molecular mass of 77.3 kDa after removal of the signal peptide. The apparent molecular mass of His-NCL is 100-130 kDa due to glycosylation.
- UniProt
- P19338
- Pathways
- Ribonucleoprotein Complex Subunit Organization
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