TIMP2 Protein (AA 27-220) (His tag)

Catalog No. ABIN7505433

Product Details

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Protein Type: Recombinant

Protein Characteristics: AA 27-220

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This TIMP2 protein is labelled with His tag.

Sequence: Cys 27-Pro 220

Characteristics: A DNA sequence encoding the Mouse TIMP-2/TIMP2 protein (P25785) (Cys 27-Pro 220) was expressed with N-His tag.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Alternative Name: TIMP-2 (TIMP2 Products)

Background:

Abbreviation: TIMP-2,TIMP2

Target Synonym: CSC-21Ktissue inhibitor of metalloproteinase 2,metalloproteinase inhibitor 2,TIMP metalloproteinase inhibitor 2,Tissue inhibitor of metalloproteinase 2.

Background: Mouse Metalloproteinase inhibitor 2(TIMP-2), belongs to a family of proteins that regulate the activation and proteolytic activity of matrix metalloproteinases (MMPs). There are four mammalian members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. The TIMP-2 is detected in testis, retina, hippocampus and cerebral cortex. The function of TIMP 2 protein is to inhibit MMPs non covalently by the formation of binary complexes. Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.And the interaction with MMP-14 facilitates the activation of pro-MMP-2.It has been shown that the binding of TIMP 2 to a3b1 integrin results in the inhibition of endothelial cell proliferation and angiogenesis.

Molecular Weight:

Calculated MW: 21.23 kDa

Observed MW: 25 kDa

UniProt: P25785

Pathways: cAMP Metabolic Process