Nerve Growth Factor Protein (NGF) (AA 122-241) (His tag)

Catalog No. ABIN7505519

Product Details

Target: Nerve Growth Factor (NGF)

Protein Type: Recombinant

Protein Characteristics: AA 122-241

Origin: Rat

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Nerve Growth Factor protein is labelled with His tag.

Sequence: Ser 122-Gly 241

Characteristics: A DNA sequence encoding the Rat Ngf protein (P25427) (Ser 122-Gly 241) was expressed with a N-His tag.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Nerve Growth Factor (NGF)

Alternative Name: Ngf (NGF Products)

Background:

Abbreviation: Ngf

Target Synonym: Beta nerve growth factor precursor,beta-nerve growth factor,betaNGF,beta-NGF,HSAN5,MGC161426,nerve growth factor (beta polypeptide),nerve growth factor,beta subunit,NGFB,NGFBMGC161428

Background: NGF is a well-characterized neurotrophic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor?dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

Molecular Weight:

Calculated MW: 13.09 kDa

Observed MW: 15 kDa

UniProt: P25427

Pathways: Regulation of Cell Size