GTF2E2 Protein (AA 1-291) (His tag)
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- Target See all GTF2E2 Proteins
- GTF2E2 (General Transcription Factor IIE, Polypeptide 2 (GTF2E2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-291
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This GTF2E2 protein is labelled with His tag.
- Purpose
- Custom-made recombinant GTF2E2 Protein expressed in mammalian cells.
- Sequence
- MDPSLLRERE LFKKRALSTP VVEKRSASSE SSSSSSKKKK TKVEHGGSSG SKQNSDHSNG SFNLKALSGS SGYKFGVLAK IVNYMKTRHQ RGDTHPLTLD EILDETQHLD IGLKQKQWLM TEALVNNPKI EVIDGKYAFK PKYNVRDKKA LLRLLDQHDQ RGLGGILLED IEEALPNSQK AVKALGDQIL FVNRPDKKKI LFFNDKSCQF SVDEEFQKLW RSVTVDSMDE EKIEEYLKRQ GISSMQESGP KKVAPIQRRK KPASQKKRRF KTHNEHLAGV LKDYSDITSS K Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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General Transcription Factor IIE, Polypeptide 2 (GTF2E2) (AA 1-292) protein (His tag)
custom-made GTF2E2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
General Transcription Factor IIE, Polypeptide 2 (GTF2E2) (AA 1-291) protein (His tag)GTF2E2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
General Transcription Factor IIE, Polypeptide 2 (GTF2E2) (AA 1-291) protein (GST tag)GTF2E2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
General Transcription Factor IIE, Polypeptide 2 (GTF2E2) (AA 1-291), (full length) protein (His tag)GTF2E2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- GTF2E2 (General Transcription Factor IIE, Polypeptide 2 (GTF2E2))
- Alternative Name
- GTF2E2 (GTF2E2 Products)
- Background
- Transcription initiation factor IIE subunit beta (TFIIE-beta) (General transcription factor IIE subunit 2),FUNCTION: Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. {ECO:0000269|PubMed:1956398, ECO:0000269|PubMed:1956404}.
- Molecular Weight
- 33.0 kDa
- UniProt
- P29084
- AlphaFold
- P29084
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