ZDHHC3 Protein (AA 1-299) (His tag)

Catalog No. ABIN7545252

Product Details

Target: ZDHHC3 (Zinc Finger, DHHC-Type Containing 3 (ZDHHC3))

Protein Type: Recombinant

Protein Characteristics: AA 1-299

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This ZDHHC3 protein is labelled with His tag.

Purpose: Custom-made recombinant ZDHHC3 Protein expressed in mammalian cells.

Sequence: MMLIPTHHFR NIERKPEYLQ PEKCVPPPYP GPVGTMWFIR DGCGIACAIV TWFLVLYAEF VVLFVMLIPS RDYVYSIING IVFNLLAFLA LASHCRAMLT DPGAVPKGNA TKEFIESLQL KPGQVVYKCP KCCSIKPDRA HHCSVCKRCI RKMDHHCPWV NNCVGENNQK YFVLFTMYIA LISLHALIMV GFHFLHCFEE DWTKCSSFSP PTTVILLILL CFEGLLFLIF TSVMFGTQVH SICTDETGIE QLKKEERRWA KKTKWMNMKA VFGHPFSLGW ASPFATPDQG KADPYQYVV Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ZDHHC3 (Zinc Finger, DHHC-Type Containing 3 (ZDHHC3))

Alternative Name: ZDHHC3 (ZDHHC3 Products)

Background: Palmitoyltransferase ZDHHC3 (EC 2.3.1.225) (Acyltransferase ZDHHC3) (EC 2.3.1.-) (Protein DHHC1) (Zinc finger DHHC domain-containing protein 3) (DHHC-3),FUNCTION: Golgi-localized palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates (PubMed:19001095, PubMed:21926431, PubMed:22240897, PubMed:23034182, PubMed:22314500). Has no stringent fatty acid selectivity and in addition to palmitate can also transfer onto target proteins myristate from tetradecanoyl-CoA and stearate from octadecanoyl-CoA (By similarity). Plays an important role in G protein-coupled receptor signaling pathways involving GNAQ and potentially other heterotrimeric G proteins by regulating their dynamic association with the plasma membrane (PubMed:19001095). Palmitoylates ITGA6 and ITGB4, thereby regulating the alpha-6/beta-4 integrin localization, expression and function in cell adhesion to laminin (PubMed:22314500). Plays a role in the TRAIL-activated apoptotic signaling pathway most probably through the palmitoylation and localization to the plasma membrane of TNFRSF10A (PubMed:22240897). In the brain, by palmitoylating the gamma subunit GABRG2 of GABA(A) receptors and regulating their postsynaptic accumulation, plays a role in synaptic GABAergic inhibitory function and GABAergic innervation (By similarity). Palmitoylates the neuronal protein GAP43 which is also involved in the formation of GABAergic synapses (By similarity). Palmitoylates NCDN thereby regulating its association with endosome membranes (By similarity). Probably palmitoylates PRCD and is involved in its proper localization within the photoreceptor (By similarity). Could mediate the palmitoylation of NCAM1 and regulate neurite outgrowth (By similarity). Could palmitoylate DNAJC5 and regulate its localization to Golgi membranes (By similarity). Also constitutively palmitoylates DLG4 (By similarity). May also palmitoylate SNAP25 (By similarity). Could palmitoylate the glutamate receptors GRIA1 and GRIA2 but this has not been confirmed in vivo (By similarity). Could also palmitoylate the D(2) dopamine receptor DRD2 (PubMed:26535572). May also palmitoylate LAMTOR1, promoting its localization to lysosomal membranes (PubMed:35893977). {ECO:0000250|UniProtKB:Q8R173, ECO:0000269|PubMed:19001095, ECO:0000269|PubMed:21926431, ECO:0000269|PubMed:22240897, ECO:0000269|PubMed:22314500, ECO:0000269|PubMed:23034182, ECO:0000269|PubMed:26535572, ECO:0000269|PubMed:35893977}., FUNCTION: May also function as a calcium transporter. {ECO:0000250|UniProtKB:Q8R173}.

Molecular Weight: 34.2 kDa

UniProt: Q9NYG2

AlphaFold: Q9NYG2