CLEC12B Protein (AA 1-276) (His tag)
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- Target See all CLEC12B Proteins
- CLEC12B (C-Type Lectin Domain Family 12, Member B (CLEC12B))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-276
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CLEC12B protein is labelled with His tag.
- Purpose
- Custom-made recombinant CLEC12B Protein expressed in mammalian cells.
- Sequence
- MSEEVTYATL TFQDSAGARN NRDGNNLRKR GHPAPSPIWR HAALGLVTLC LMLLIGLVTL GMMFLQISND INSDSEKLSQ LQKTIQQQQD NLSQQLGNSN NLSMEEEFLK SQISSVLKRQ EQMAIKLCQE LIIHTSDHRC NPCPKMWQWY QNSCYYFTTN EEKTWANSRK DCIDKNSTLV KIDSLEEKDF LMSQPLLMFS FFWLGLSWDS SGRSWFWEDG SVPSPSLFST KELDQINGSK GCAYFQKGNI YISRCSAEIF WICEKTAAPV KTEDLD Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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C-Type Lectin Domain Family 12, Member B (CLEC12B) (AA 1-275) protein (His tag)
custom-made CLEC12B Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
C-Type Lectin Domain Family 12, Member B (CLEC12B) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)CLEC12B Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- CLEC12B (C-Type Lectin Domain Family 12, Member B (CLEC12B))
- Alternative Name
- CLEC12B (CLEC12B Products)
- Background
- C-type lectin domain family 12 member B (Macrophage antigen H),FUNCTION: Inhibitory receptor postulated to negatively regulate immune and non-immune functions (PubMed:17562706, PubMed:34310951). Upon phosphorylation, recruits SH2 domain-containing PTPN6 and PTPN11 phosphatases to its ITIM motif and antagonizes activation signals (PubMed:17562706, PubMed:34310951). Although it inhibits KLRK1/NKG2D-mediated signaling, it does not bind known ligands of KLRK1/NKG2D and therefore is not its inhibitory counterpart (PubMed:17562706). May limit activation of myeloid cell subsets in response to infection or tissue inflammation (PubMed:17562706). May protect target cells against natural killer cell-mediated lysis (PubMed:17562706). May negatively regulate cell cycle and differentiation of melanocytes via inactivation of STAT3 (PubMed:34310951). {ECO:0000269|PubMed:17562706, ECO:0000269|PubMed:34310951}.
- Molecular Weight
- 31.6 kDa
- UniProt
- Q2HXU8
- AlphaFold
- Q2HXU8
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