DDX52 Protein (AA 1-599) (His tag)
custom-made
DDX52
Origin: Human
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all DDX52 products
- DDX52 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 52 (DDX52))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-599
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This DDX52 protein is labelled with His tag.
- Purpose
- Custom-made recombinant DDX52 Protein expressed in mammalian cells.
- Sequence
- MDVHDLFRRL GAGAKFDTRR FSADAARFQI GKRKYDFDSS EVLQGLDFFG NKKSVPGVCG ASQTHQKPQN GEKKEESLTE RKREQSKKKR KTMTSEIASQ EEGATIQWMS SVEAKIEDKK VQRESKLTSG KLENLRKEKI NFLRNKHKIH VQGTDLPDPI ATFQQLDQEY KINSRLLQNI LDAGFQMPTP IQMQAIPVML HGRELLASAP TGSGKTLAFS IPILMQLKQP ANKGFRALII SPTRELASQI HRELIKISEG TGFRIHMIHK AAVAAKKFGP KSSKKFDILV TTPNRLIYLL KQDPPGIDLA SVEWLVVDES DKLFEDGKTG FRDQLASIFL ACTSHKVRRA MFSATFAYDV EQWCKLNLDN VISVSIGARN SAVETVEQEL LFVGSETGKL LAVRELVKKG FNPPVLVFVQ SIERAKELFH ELIYEGINVD VIHAERTQQQ RDNTVHSFRA GKIWVLICTA LLARGIDFKG VNLVINYDFP TSSVEYIHRI GRTGRAGNKG KAITFFTEDD KPLLRSVANV IQQAGCPVPE YIKGFQKLLS KQKKKMIKKP LERESISTTP KCFLEKAKDK QKKVTGQNSK KKVALEDKS Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 52 (DDX52) (AA 1-598) protein (His tag)
custom-made DDX52 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- DDX52 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 52 (DDX52))
- Alternative Name
- DDX52 (DDX52 Products)
- Background
- Probable ATP-dependent RNA helicase DDX52 (EC 3.6.4.13) (ATP-dependent RNA helicase ROK1-like) (DEAD box protein 52),FUNCTION: Required for efficient ribosome biogenesis (By similarity). May control cell cycle progression by regulating translation of mRNAs that contain a terminal oligo pyrimidine (TOP) motif in their 5' UTRs, such as GTPBP4 (By similarity). {ECO:0000250|UniProtKB:Q9VVK8}.
- Molecular Weight
- 67.5 kDa
- UniProt
- Q9Y2R4
- AlphaFold
- Q9Y2R4
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