GPR171 Protein (AA 1-319) (His tag)
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- Target See all GPR171 Proteins
- GPR171 (G Protein-Coupled Receptor 171 (GPR171))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-319
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This GPR171 protein is labelled with His tag.
- Purpose
- Custom-made recombinant GPR171 Protein expressed in mammalian cells.
- Sequence
- MTNSSFFCPV YKDLEPFTYF FYLVFLVGII GSCFATWAFI QKNTNHRCVS IYLINLLTAD FLLTLALPVK IVVDLGVAPW KLKIFHCQVT ACLIYINMYL SIIFLAFVSI DRCLQLTHSC KIYRIQEPGF AKMISTVVWL MVLLIMVPNM MIPIKDIKEK SNVGCMEFKK EFGRNWHLLT NFICVAIFLN FSAIILISNC LVIRQLYRNK DNENYPNVKK ALINILLVTT GYIICFVPYH IVRIPYTLSQ TEVITDCSTR ISLFKAKEAT LLLAVSNLCF DPILYYHLSK AFRSKVTETF ASPKETKAQK EKLRCENNA Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
- Top Product
- Discover our top product GPR171 Protein
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G Protein-Coupled Receptor 171 (GPR171) (AA 1-319) protein (His tag)
custom-made GPR171 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
G Protein-Coupled Receptor 171 (GPR171) protein (Myc-DYKDDDDK Tag)GPR171 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- GPR171 (G Protein-Coupled Receptor 171 (GPR171))
- Alternative Name
- GPR171 (GPR171 Products)
- Background
- G-protein coupled receptor 171 (G-protein coupled receptor H963),FUNCTION: G-protein coupled receptor for Big LEN, a 16-amino acid neuropeptide produced from the precursor protein, proSAAS (encoded by PCSK1N). Acts through a G(i)-alpha-mediated pathway in response to Big LEN. Big LEN-GPR171 system plays an important role in regulating feeding and metabolism. Also plays a role in modulating fear and anxiety-like behaviors in the basolateral amygdala. Big LEN-GPR171 modulates the mu-type opioid receptor signaling and antinociception (By similarity). Acts as a negative regulator T cell function (PubMed:34615877). {ECO:0000250|UniProtKB:Q8BG55, ECO:0000269|PubMed:34615877}.
- Molecular Weight
- 36.8 kDa
- UniProt
- O14626
- AlphaFold
- O14626
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