PTPLAD2 Protein (AA 1-232) (His tag)
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- Target See all PTPLAD2 products
- PTPLAD2 (Protein tyrosine Phosphatase-Like A Domain Containing 2 (PTPLAD2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-232
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PTPLAD2 protein is labelled with His tag.
- Purpose
- Custom-made recombinant HACD4 Protein expressed in mammalian cells.
- Sequence
- MGPLALPAWL QPRYRKNAYL FIYYLIQFCG HSWIFTNMTV RFFSFGKDSM VDTFYAIGLV MRLCQSVSLL ELLHIYVGIE SNHLLPRFLQ LTERIIILFV VITSQEEVQE KYVVCVLFVF WNLLDMVRYT YSMLSVIGIS YAVLTWLSQT LWMPIYPLCV LAEAFAIYQS LPYFESFGTY STKLPFDLSI YFPYVLKIYL MMLFIGMYFT YSHLYSERRD ILGIFPIKKK KM Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- PTPLAD2 (Protein tyrosine Phosphatase-Like A Domain Containing 2 (PTPLAD2))
- Alternative Name
- HACD4 (PTPLAD2 Products)
- Background
- Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 4 (EC 4.2.1.134) (3-hydroxyacyl-CoA dehydratase 4) (HACD4) (Protein-tyrosine phosphatase-like A domain-containing protein 2),FUNCTION: Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. {ECO:0000269|PubMed:18554506}.
- Molecular Weight
- 27.5 kDa
- UniProt
- Q5VWC8
- AlphaFold
- Q5VWC8
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