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PTPLAD2 Protein (AA 1-232) (His tag)

custom-made PTPLAD2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Catalog No. ABIN7548214
  • Target See all PTPLAD2 products
    PTPLAD2 (Protein tyrosine Phosphatase-Like A Domain Containing 2 (PTPLAD2))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-232
    Origin
    • 2
    • 1
    Human
    Source
    • 2
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This PTPLAD2 protein is labelled with His tag.
    Purpose
    Custom-made recombinant HACD4 Protein expressed in mammalian cells.
    Sequence
    MGPLALPAWL QPRYRKNAYL FIYYLIQFCG HSWIFTNMTV RFFSFGKDSM VDTFYAIGLV MRLCQSVSLL ELLHIYVGIE SNHLLPRFLQ LTERIIILFV VITSQEEVQE KYVVCVLFVF WNLLDMVRYT YSMLSVIGIS YAVLTWLSQT LWMPIYPLCV LAEAFAIYQS LPYFESFGTY STKLPFDLSI YFPYVLKIYL MMLFIGMYFT YSHLYSERRD ILGIFPIKKK KM Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Specificity
    If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
    Characteristics
    Key Benefits:
    • Made to order protein - from design to production - by highly experienced protein experts.
    • Protein expressed in mammalian cells and purified in one-step affinity chromatography
    • The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).
    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    If you are not interested in a full length protein, please contact us for individual protein fragments.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
    Purity
    > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
    Grade
    custom-made
  • Application Notes
    We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    12 months
  • Target
    PTPLAD2 (Protein tyrosine Phosphatase-Like A Domain Containing 2 (PTPLAD2))
    Alternative Name
    HACD4 (PTPLAD2 Products)
    Background
    Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 4 (EC 4.2.1.134) (3-hydroxyacyl-CoA dehydratase 4) (HACD4) (Protein-tyrosine phosphatase-like A domain-containing protein 2),FUNCTION: Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. {ECO:0000269|PubMed:18554506}.
    Molecular Weight
    27.5 kDa
    UniProt
    Q5VWC8
    AlphaFold
    Q5VWC8
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